منابع مشابه
Rapid fixed-time assay for penicillinase.
Many methods of assaying penicillinase activity have been devised (N. Citri and M. R. Pollock, Advan. Enzymol. 28:237, 1966; N. Citri, Methods Med. Res., 10:221, 1964; J. M. T. HamiltonMiller et al., J. Pharm. Pharmacol. 15:81, 1963; D. A. Wolfe and M. Hamberger, J. Lab. Clin. Med. 59:469, 1962). These have included colorimetric determinations of acid or alkali production, manometric techniques...
متن کاملNew Assay for Penicillinase and Some Results on Penicillinase Induction.
Imsande, John (Western Reserve University, Cleveland, Ohio). New assay for penicillinase and some results on penicillinase induction. J. Bacteriol. 89:1322-1327. 1965.-A rapid, sensitive, and reliable assay of penicillinase activity is described. Studies conducted on the induction of penicillinase, with use of the new assay, show that actinomycin D inhibits growth and the induced synthesis of p...
متن کاملENZYME-LINKED IMMUNOSORBENT ASSAY OF NEOPTERIN USING PENICILLINASE AS LABEL
An enzyme-linked immunosorbent assay for neopterin using penicillinase as marker enzyme is reported here by polyclonal antibodies against neopterin conjugated to bovine serum albumin which were raised in rabbits. Immunoglobulin fractions were purified and coated on wells of microtiter plates. A chain heterology was introduced in neopterin derivative and conjugated to penicillinase. The assa...
متن کاملTechniques for the rapid and sensitive detection of penicillinase.
Practical problems in the detection of penicillinase are discussed. A membrane technique for this purpose is described, with various modifications, suitable for screening large numbers of organisms and yet more sensitive than most methods.A one-stage membrane technique is adequate for detecting hydrolysis of penicillin G but a two-stage technique is required to demonstrate hydrolysis of some ot...
متن کاملCholinesterase assay by an efficient fixed time endpoint method
Many cholinesterase assays are performed to study the inhibition of cholinesterase (ChE) activity. Frequently a large number of samples are processed and Ellman's method [1] is the most commonly used [2,3]. Activity is estimated from the increment in absorbance between two reaction times when the reaction is not stopped. Bellino et al. [4] described a method based on Ellman's method whereby the...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1968
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.95.4.1493-1494.1968